diff --git a/tests/validation_new/NEON/BitwiseNot.cpp b/tests/validation_new/NEON/BitwiseNot.cpp
new file mode 100644
index 0000000..3725379
--- /dev/null
+++ b/tests/validation_new/NEON/BitwiseNot.cpp
@@ -0,0 +1,90 @@
+/*
+ * Copyright (c) 2017 ARM Limited.
+ *
+ * SPDX-License-Identifier: MIT
+ *
+ * Permission is hereby granted, free of charge, to any person obtaining a copy
+ * of this software and associated documentation files (the "Software"), to
+ * deal in the Software without restriction, including without limitation the
+ * rights to use, copy, modify, merge, publish, distribute, sublicense, and/or
+ * sell copies of the Software, and to permit persons to whom the Software is
+ * furnished to do so, subject to the following conditions:
+ *
+ * The above copyright notice and this permission notice shall be included in all
+ * copies or substantial portions of the Software.
+ *
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+ * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+ * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+ * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+ * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+ * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
+ * SOFTWARE.
+ */
+#include "arm_compute/core/Types.h"
+#include "arm_compute/runtime/NEON/functions/NEBitwiseNot.h"
+#include "arm_compute/runtime/Tensor.h"
+#include "arm_compute/runtime/TensorAllocator.h"
+#include "framework/Asserts.h"
+#include "framework/Macros.h"
+#include "framework/datasets/Datasets.h"
+#include "tests/NEON/Accessor.h"
+#include "tests/PaddingCalculator.h"
+#include "tests/datasets_new/ShapeDatasets.h"
+#include "tests/validation_new/Validation.h"
+#include "tests/validation_new/fixtures/BitwiseNotFixture.h"
+
+namespace arm_compute
+{
+namespace test
+{
+namespace validation
+{
+TEST_SUITE(NEON)
+TEST_SUITE(BitwiseNot)
+
+DATA_TEST_CASE(Configuration, framework::DatasetMode::ALL, combine(concat(datasets::SmallShapes(), datasets::LargeShapes()), framework::dataset::make("DataType", DataType::U8)), shape, data_type)
+{
+    // Create tensors
+    Tensor src = create_tensor<Tensor>(shape, data_type);
+    Tensor dst = create_tensor<Tensor>(shape, data_type);
+
+    ARM_COMPUTE_EXPECT(src.info()->is_resizable(), framework::LogLevel::ERRORS);
+    ARM_COMPUTE_EXPECT(dst.info()->is_resizable(), framework::LogLevel::ERRORS);
+
+    // Create and configure function
+    NEBitwiseNot bitwise_not;
+    bitwise_not.configure(&src, &dst);
+
+    // Validate valid region
+    const ValidRegion valid_region = shape_to_valid_region(shape);
+    validate(src.info()->valid_region(), valid_region);
+    validate(dst.info()->valid_region(), valid_region);
+
+    // Validate padding
+    const PaddingSize padding = PaddingCalculator(shape.x(), 16).required_padding();
+    validate(src.info()->padding(), padding);
+    validate(dst.info()->padding(), padding);
+}
+
+template <typename T>
+using NEBitwiseNotFixture = BitwiseNotValidationFixture<Tensor, Accessor, NEBitwiseNot, T>;
+
+FIXTURE_DATA_TEST_CASE(RunSmall, NEBitwiseNotFixture<uint8_t>, framework::DatasetMode::PRECOMMIT, combine(datasets::SmallShapes(), framework::dataset::make("DataType",
+                                                                                                          DataType::U8)))
+{
+    // Validate output
+    validate(Accessor(_target), _reference);
+}
+FIXTURE_DATA_TEST_CASE(RunLarge, NEBitwiseNotFixture<uint8_t>, framework::DatasetMode::NIGHTLY, combine(datasets::LargeShapes(), framework::dataset::make("DataType",
+                                                                                                        DataType::U8)))
+{
+    // Validate output
+    validate(Accessor(_target), _reference);
+}
+
+TEST_SUITE_END()
+TEST_SUITE_END()
+} // namespace validation
+} // namespace test
+} // namespace arm_compute