diff --git a/tests/validation/dynamic_fusion/gpu/cl/Sub.cpp b/tests/validation/dynamic_fusion/gpu/cl/Sub.cpp
new file mode 100644
index 0000000..977e011
--- /dev/null
+++ b/tests/validation/dynamic_fusion/gpu/cl/Sub.cpp
@@ -0,0 +1,259 @@
+/*
+ * Copyright (c) 2023 Arm Limited.
+ *
+ * SPDX-License-Identifier: MIT
+ *
+ * Permission is hereby granted, free of charge, to any person obtaining a copy
+ * of this software and associated documentation files (the "Software"), to
+ * deal in the Software without restriction, including without limitation the
+ * rights to use, copy, modify, merge, publish, distribute, sublicense, and/or
+ * sell copies of the Software, and to permit persons to whom the Software is
+ * furnished to do so, subject to the following conditions:
+ *
+ * The above copyright notice and this permission notice shall be included in all
+ * copies or substantial portions of the Software.
+ *
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+ * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+ * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+ * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+ * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+ * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
+ * SOFTWARE.
+ */
+
+#include "arm_compute/dynamic_fusion/sketch/gpu/GpuWorkloadSketch.h"
+#include "arm_compute/dynamic_fusion/sketch/gpu/operators/GpuSub.h"
+
+#include "tests/CL/CLAccessor.h"
+#include "tests/framework/Fixture.h"
+#include "tests/framework/Macros.h"
+#include "tests/framework/datasets/Datasets.h"
+#include "tests/validation/Validation.h"
+
+#include "tests/datasets/DynamicFusionDataset.h"
+#include "tests/datasets/ShapeDatasets.h"
+#include "tests/validation/fixtures/dynamic_fusion/gpu/cl/ElementwiseBinaryFixture.h"
+
+namespace arm_compute
+{
+namespace test
+{
+namespace validation
+{
+/* Synced with tests/validation/CL/ArithmeticSubtraction.cpp from the standard interface.
+ *
+ * Difference          | Why the difference
+ * No quantized tests  | Not supported yet
+ * No in place tests   | Not supported yet
+ * No activation tests | Not needed in dynamic fusion interface
+ *
+ */
+TEST_SUITE(CL)
+TEST_SUITE(DYNAMIC_FUSION)
+TEST_SUITE(SUB)
+
+// *INDENT-OFF*
+// clang-format off
+DATA_TEST_CASE(Validate, framework::DatasetMode::ALL, zip(zip(
+               framework::dataset::make("LhsInfo", { TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::U32),    // Unsupported data type U32
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::QASYMM8),    // Unsupported data type QASYMM8
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::QASYMM8_SIGNED),    // Unsupported data type QASYMM8
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),    // Invalid data type combination
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::S16),    // S16 is valid data type for Sub
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::S32),    // S32 is valid data type for Sub
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),    // Mismatching shapes
+                                                        TensorInfo(TensorShape(32U,  1U, 1U), 1, DataType::F32),    // Broadcasting allowed for lhs
+                                                        TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),
+                                                        TensorInfo(TensorShape(15U, 23U, 3U), 1, DataType::F32),    // Broadcast Y dimension is not allowed
+                                                        TensorInfo(TensorShape( 3U,  8U, 9U), 1, DataType::S16),    // Broadcast Z dimension is not allowed
+                                                        TensorInfo(TensorShape(32U, 13U, 2U, 2), 1, DataType::F32), // Batching is allowed
+                                                      }),
+               framework::dataset::make("RhsInfo",{ TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::U32),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::QASYMM8),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::QASYMM8_SIGNED),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F16),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::S16),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::S32),
+                                                       TensorInfo(TensorShape(48U, 11U, 2U), 1, DataType::F32),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U), 1, DataType::F32),
+                                                       TensorInfo(TensorShape(32U,  1U, 1U), 1, DataType::F32),    // Broadcasting allowed for rhs
+                                                       TensorInfo(TensorShape(15U,  1U, 3U), 1, DataType::F32),
+                                                       TensorInfo(TensorShape( 3U,  8U, 1U), 1, DataType::S16),
+                                                       TensorInfo(TensorShape(32U, 13U, 2U, 2), 1, DataType::F32),
+                                                      })),
+               framework::dataset::make("Expected", { true, false, false, false, false, true, true, false, true, true, false, false, true })),
+               input1_info, input2_info, expected)
+{
+    // Create a new workload sketch
+    auto              cl_compile_ctx = CLKernelLibrary::get().get_compile_context();
+    auto              gpu_ctx        = GpuWorkloadContext{ &cl_compile_ctx };
+    GpuWorkloadSketch sketch{ &gpu_ctx };
+
+    // Validate Elementwise Sub
+    auto          lhs_info         = sketch.create_tensor_info(input1_info);
+    auto          rhs_info         = sketch.create_tensor_info(input2_info);
+
+    bool res = bool(GpuSub::validate_op(sketch, &lhs_info, &rhs_info));
+    ARM_COMPUTE_EXPECT(res == expected, framework::LogLevel::ERRORS);
+}
+// clang-format on
+// *INDENT-ON*
+
+template <typename T>
+using DynamicFusionCLSubFixture = DynamicFusionGpuElementwiseBinaryOneOpValidationFixture<CLTensor, CLAccessor, GpuSub, T>;
+
+template <typename T>
+using DynamicFusionCLSubBroadcastFixture = DynamicFusionGpuElementwiseBinaryBroadcastOneOpValidationFixture<CLTensor, CLAccessor, GpuSub, T>;
+
+template <typename T>
+using DynamicFusionCLSubTwoOpsFixture = DynamicFusionGpuElementwiseBinaryTwoOpsValidationFixture<CLTensor, CLAccessor, GpuSub, T>;
+
+TEST_SUITE(FP32)
+FIXTURE_DATA_TEST_CASE(RunSmallOneOp,
+                       DynamicFusionCLSubFixture<float>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::SmallShapes()),
+                                       framework::dataset::make("DataType", { DataType::F32 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+FIXTURE_DATA_TEST_CASE(RunLargeOneOp,
+                       DynamicFusionCLSubFixture<float>,
+                       framework::DatasetMode::NIGHTLY,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::LargeShapes()),
+                                       framework::dataset::make("DataType", { DataType::F32 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+FIXTURE_DATA_TEST_CASE(RunSmallBroadcastOneOp,
+                       DynamicFusionCLSubBroadcastFixture<float>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::TemporaryLimitedSmallShapesBroadcast()),
+                                       framework::dataset::make("DataType", { DataType::F32 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+
+FIXTURE_DATA_TEST_CASE(RunLargeBroadcastOneOp,
+                       DynamicFusionCLSubBroadcastFixture<float>,
+                       framework::DatasetMode::NIGHTLY,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::TemporaryLimitedLargeShapesBroadcast()),
+                                       framework::dataset::make("DataType", { DataType::F32 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+FIXTURE_DATA_TEST_CASE(RunSmallTwoOps,
+                       DynamicFusionCLSubTwoOpsFixture<float>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                                       datasets::DynamicFusionElementwiseBinaryTwoOpsSmallShapes()),
+                                               framework::dataset::make("DataType", { DataType::F32 })),
+                                       framework::dataset::make("InPlace", { false })),
+                               framework::dataset::make("FuseTwoOps", { true })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+TEST_SUITE_END() // FP32
+
+TEST_SUITE(FP16)
+FIXTURE_DATA_TEST_CASE(RunSmallOneOp,
+                       DynamicFusionCLSubFixture<half>,
+                       framework::DatasetMode::ALL,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::SmallShapes()),
+                                       framework::dataset::make("DataType", { DataType::F16 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+
+FIXTURE_DATA_TEST_CASE(RunSmallBroadcastOneOp,
+                       DynamicFusionCLSubBroadcastFixture<half>,
+                       framework::DatasetMode::ALL,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::TemporaryLimitedSmallShapesBroadcast()),
+                                       framework::dataset::make("DataType", { DataType::F16 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+
+TEST_SUITE_END() // FP16
+
+TEST_SUITE(S32)
+FIXTURE_DATA_TEST_CASE(RunSmall,
+                       DynamicFusionCLSubFixture<int32_t>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::SmallShapes()),
+                                       framework::dataset::make("DataType", { DataType::S32 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+TEST_SUITE_END() // S32
+
+TEST_SUITE(S16)
+FIXTURE_DATA_TEST_CASE(RunSmall,
+                       DynamicFusionCLSubFixture<int16_t>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::SmallShapes()),
+                                       framework::dataset::make("DataType", { DataType::S16 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+FIXTURE_DATA_TEST_CASE(RunLarge,
+                       DynamicFusionCLSubFixture<int16_t>,
+                       framework::DatasetMode::NIGHTLY,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::LargeShapes()),
+                                       framework::dataset::make("DataType", { DataType::S16 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+TEST_SUITE_END() // S16
+
+TEST_SUITE(U8)
+FIXTURE_DATA_TEST_CASE(RunSmall,
+                       DynamicFusionCLSubFixture<uint8_t>,
+                       framework::DatasetMode::PRECOMMIT,
+                       combine(combine(combine(framework::dataset::make("ElementwiseOp", { ArithmeticOperation::SUB }),
+                                               datasets::SmallShapes()),
+                                       framework::dataset::make("DataType", { DataType::U8 })),
+                               framework::dataset::make("InPlace", { false })))
+{
+    // Validate output
+    validate(CLAccessor(_target), _reference);
+}
+TEST_SUITE_END() // U8
+
+TEST_SUITE_END() // SUB
+TEST_SUITE_END() // DYNAMIC_FUSION
+TEST_SUITE_END() // CL
+} // namespace validation
+} // namespace test
+} // namespace arm_compute